Differences in DNA methylation profiles by histologic subtype of paediatric germ cell tumours: a report from the Children's Oncology Group.

ABSTRACT:

Background

Abnormal DNA methylation may be important in germ cell tumour (GCT) aetiology, as germ cells undergo complete epigenetic reprogramming during development. GCTs show differences in global and promoter methylation patterns by histologic subtype. We conducted an epigenome-wide study to identify methylation differences by GCT histology.

Methods

Using the Illumina HumanMethylation450K array we measured methylation in 154 paediatric GCTs (21 germinomas/seminomas/dysgerminoma, 70 yolk sac tumours [YST], 9 teratomas, and 54 mixed histology tumours). We identified differentially methylated regions (DMRs) between GCT histologies by comparing methylation beta values.

Results

We identified 8,481 DMRs (FWER < 0.05). Unsupervised hierarchical clustering of individual pro

SUBMITTER: Williams LA 

PROVIDER: S-EPMC6189207 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature