Neoantigenic Potential of Complex Chromosomal Rearrangements in Mesothelioma.

ABSTRACT:

Introduction

Malignant pleural mesothelioma is a disease primarily associated with exposure to the carcinogen asbestos. Whereas other carcinogen-related tumors are associated with a high tumor mutation burden, mesothelioma is not. We sought to resolve this discrepancy.

Methods

We used mate-pair (n = 22), RNA (n = 28), and T cell receptor sequencing along with in silico predictions and immunologic assays to understand how structural variants of chromosomes affect the transcriptome.

Results

We observed that inter- or intrachromosomal rearrangements were present in every specimen and were frequently in a pattern of chromoanagenesis such as chromoplexy or chromothripsis. Transcription of rearrangement-related junctions was predicted to result in many potential neoantigens

SUBMITTER: Mansfield AS 

PROVIDER: S-EPMC6348045 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature