A rapid and sensitive recombinase aided amplification assay to detect hepatitis B virus without DNA extraction.

ABSTRACT:

Background

Hepatitis B virus (HBV) infection is the major public health problem worldwide. In clinical practice, serological and molecular assays are the most commonly used diagnostic methods to detect HBV infection in clinical practices.

Methods

Here we present a rapid and sensitive recombinase aided amplification assay (RAA) to detect HBV at 39.0 °C for 30 min without DNA extraction from serum samples. The analytical sensitivity of RAA assay was 100 copies per reaction and showed no cross reaction with human immunodeficiency virus (HIV) and hepatitis C virus (HCV). The universality of RAA assay was validated by testing of 41 archived serum samples with predefined HBV genotypes (B, C and D).

Results

A total of 130 archived suspected HBV infected serum samples were de

SUBMITTER: Shen XX 

PROVIDER: S-EPMC6402085 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature