Development of a real-time recombinase-aided amplification assay for rapid and sensitive detection of Edwardsiella piscicida.

ABSTRACT: Edwardsiella piscicida, a significant intracellular pathogen, is widely distributed in aquatic environments and causes systemic infection in various species. Therefore, it's essential to develop a rapid, uncomplicated and sensitive method for detection of E. piscicida in order to control the transmission of this pathogen effectively. The recombinase-aided amplification (RAA) assay is a newly developed, rapid detection method that has been utilized for various pathogens. In the present study, a real-time RAA (RT-RAA) assay, targeting the conserved positions of the EvpP gene, was successfully established for the detection of E. piscicida. This assay can be performed in a one-step single tube reaction at a temperature of 39°C within 20 min. The RT-RAA assay exhibit

SUBMITTER: Dong Y 

PROVIDER: S-EPMC11007066 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature