The Effects of Immunosuppressive Factors on Primary Dendritic Cells from C57BL/6 and CBA Mice.

ABSTRACT:

Introduction

Dendritic cells (DCs) control immune responses by modulating T and B cells towards effector or tolerogenic responses. In this study, we evaluated the effects of different immunosuppressive molecules on the phenotypic and functional characteristics of primary dendritic cells from C57BL/6 and CBA mice.

Methods

DCs were derived from bone marrow cells in the presence of rmGM-CSF and rmIL-4. DCs were then treated with different types of immunosuppressive molecules (rmIL-10, rmTGF-β, and BAY 11-7082) and cocultured with syngeneic splenocytes. The amount of CD4+CD25hiFoxP3+ Tregs, IL-10 expression, and proliferation were evaluated.

Results

Tolerogenic factors were found to have different effects on DCs C57Bl/6 mice. In C57Bl/6 mice, BAY 11-7082 alone had

SUBMITTER: Kurilin VV 

PROVIDER: S-EPMC6501430 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature