CarD contributes to diverse gene expression outcomes throughout the genome of Mycobacterium tuberculosis.

ABSTRACT: The ability to regulate gene expression through transcription initiation underlies the adaptability and survival of all bacteria. Recent work has revealed that the transcription machinery in many bacteria diverges from the paradigm that has been established in Escherichia coliMycobacterium tuberculosis (Mtb) encodes the RNA polymerase (RNAP)-binding protein CarD, which is absent in E. coli but is required to form stable RNAP-promoter open complexes (RP_o) and is essential for viability in Mtb The stabilization of RP_o by CarD has been proposed to result in activation of gene expression; however, CarD has only been examined on limited promoters that do not represent the typical promoter structure in Mtb In this study, we investig

SUBMITTER: Zhu DX 

PROVIDER: S-EPMC6613185 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature