Extensive post-transcriptional buffering of gene expression in the response to severe oxidative stress in baker's yeast.

ABSTRACT: Cells responds to diverse stimuli by changing the levels of specific effector proteins. These changes are usually examined using high throughput RNA sequencing data (RNA-Seq); transcriptional regulation is generally assumed to directly influence protein abundances. However, the correlation between RNA-Seq and proteomics data is in general quite limited owing to differences in protein stability and translational regulation. Here we perform RNA-Seq, ribosome profiling and proteomics analyses in baker's yeast cells grown in rich media and oxidative stress conditions to examine gene expression regulation at various levels. With the exception of a small set of genes involved in the maintenance of the redox state, which are regulated at the transcriptional level, modulation of protein expression

SUBMITTER: Blevins WR 

PROVIDER: S-EPMC6662803 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature