Early detection of unilateral ureteral obstruction by desorption electrospray ionization mass spectrometry.

ABSTRACT: Desorption electrospray ionization mass spectrometry (DESI-MS) is an emerging analytical tool for rapid in situ assessment of metabolomic profiles on tissue sections without tissue pretreatment or labeling. We applied DESI-MS to identify candidate metabolic biomarkers associated with kidney injury at the early stage. DESI-MS was performed on sections of kidneys from 80 mice over a time course following unilateral ureteral obstruction (UUO) and compared to sham controls. A predictive model of renal damage was constructed using the LASSO (least absolute shrinkage and selection operator) method. Levels of lipid and small metabolites were significantly altered and glycerophospholipids comprised a significant fraction of altered species. These changes correlate with altered expression of lipid

SUBMITTER: Banerjee S 

PROVIDER: S-EPMC6662848 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature