IL-10-Dependent Crosstalk between Murine Marginal Zone B Cells, Macrophages, and CD8α⁺ Dendritic Cells Promotes Listeria monocytogenes Infection.

ABSTRACT: Type 1 CD8α⁺ conventional dendritic cells (cDC1s) are required for CD8⁺ T cell priming but, paradoxically, promote splenic Listeria monocytogenes infection. Using mice with impaired cDC2 function, we ruled out a role for cDC2s in this process and instead discovered an interleukin-10 (IL-10)-dependent cellular crosstalk in the marginal zone (MZ) that promoted bacterial infection. Mice lacking the guanine nucleotide exchange factor DOCK8 or CD19 lost IL-10-producing MZ B cells and were resistant to Listeria. IL-10 increased intracellular Listeria in cDC1s indirectly by reducing inducible nitric oxide synthase expression early after infection and increasing intracellular Listeria in MZ metallophilic macrophages (MMMs). These MMMs trans-infected cDC1s, which, in turn, tra

SUBMITTER: Liu D 

PROVIDER: S-EPMC6685086 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature