Development of a high-throughput ?-H2AX assay based on imaging flow cytometry.

ABSTRACT:

Background

Measurement of ?-H2AX foci levels in cells provides a sensitive and reliable method for quantitation of the radiation-induced DNA damage response. The objective of the present study was to develop a rapid, high-throughput ?-H2AX assay based on imaging flow cytometry (IFC) using the ImageStream®^X Mk II (ISX) platform to evaluate DNA double strand break (DSB) repair kinetics in human peripheral blood cells after exposure to ionizing irradiation.

Methods

The ?-H2AX protocol was developed and optimized for small volumes (100??L) of human blood in Matrix™ 96-tube format. Blood cell lymphocytes were identified and captured by ISX INSPIRE™ software and analyzed by Data Exploration and Analysis Software.

Results

Dose- and time-dependent ?-H2AX levels corresponding to radiation exposure were measured at various time points over 24?h using the IFC system. ?-H2AX fluorescence intensity at 1?h after exposure, increased linearly with increasing radiation dose (R² =?0.98) for the four human donors tested, whereas the dose response for the mean number of ?-H2AX foci/cell was not as robust (R² =?0.81). Radiation-induced ?-H2AX levels rapidly increased within 30?min and reached a maximum by ~?1?h, after which time there was fast decline by 6?h, followed by a much slower rate of disappearance up to 24?h. A mathematical approach for quantifying DNA repair kinetics using the rate of ?-H2AX decay (decay constant, K_dec), and yield of residual unrepaired breaks (F_res) demonstrated differences in individual repair capacity between the healthy donors.

Conclusions

The results indicate that the IFC-based ?-H2AX protocol may provide a practical and high-throughput platform for measurements of individual global DNA DSB repair capacity which can facilitate precision medicine by predicting individual radiosensitivity and risk of developing adverse effects related to radiotherapy treatment.

SUBMITTER: Lee Y 

PROVIDER: S-EPMC6704696 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature