High-throughput detection and quantification of mitochondrial fusion through imaging flow cytometry.

ABSTRACT: Mitochondria are highly dynamic organelles whose fusion and fission play an increasingly important role in a number of both normal and pathological cellular functions. Despite the increased interest in mitochondrial dynamics, robust, and quantitative methods to analyze mitochondrial fusion and fission activity in intact cells have not been developed. The current state-of-the art method to measure mitochondrial fusion activity is the polyethylene glycol (PEG) fusion assay in which cells expressing distinct mitochondrially-targeted fluorescent proteins (FPs) are fused together and mitochondrial fusion activity is determined by the rate at which color mixing occurs. Although this assay is useful, cell-cell fusion events are rare, and finding the number of fused cells required to generate stat

SUBMITTER: Nascimento A 

PROVIDER: S-EPMC5503215 | biostudies-literature | 2016 Aug

REPOSITORIES: biostudies-literature