Dataset Information

Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

ABSTRACT:

Purpose

Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model.

Methods

Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antiangiogenic peptides were used to block the angiogenic effect of CML-derived exosomes, assessed by evaluation of expression levels of key modulators involved in angiogenic pathways - VEGFA, VEGFR1 (also known as FLT1) and IL8.

Results

Exosomes isolated from K562 cells promoted the doubling of newly formed vessels associated with the increase of VEGFR1 expression. This is a concentration and time-dependent effect. The AuNPs functionalized with antiangiogenic peptides were capable to block the angiogenic effect by modulating VEGFR1 associated pathway.

Conclusion

Exosomes derived from blast cells are capable to trigger (neo)-angiogenesis, a key factor for the progression and spreading of cancer, in particular in CML. AuNPs functionalized with specific antiangiogenic peptides are capable to block the effect of the exosomes produced by malignant cells via modulation of the intrinsic VEGFR pathway. Together, these data highlight the potential of nanomedicine-based strategies against cancer proliferation.

SUBMITTER: Roma-Rodrigues C

PROVIDER: S-EPMC6716571 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

Roma-Rodrigues Catarina C Fernandes Alexandra R AR Baptista Pedro V PV

International journal of nanomedicine 20190826

<h4>Purpose</h4>Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model.<h4>Methods</h4>Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antian ...[more]

PMID: 31692567

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Dataset Information

Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

Purpose

Methods

Results

Conclusion

Publications

Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

Similar Datasets

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