Counteracting the effect of leukemia exosomes by antiangiogenic gold nanoparticles.

ABSTRACT:

Purpose

Progression of chronic myeloid leukemia (CML) is frequently associated with increased angiogenesis at the bone marrow mediated by exosomes. The capability of gold nanoparticles (AuNPs) functionalized with antiangiogenic peptides to hinder the formation of new blood vessels has been demonstrated in a chorioallantoic membrane (CAM) model.

Methods

Exosomes of K562 CML cell line were isolated and their angiogenic effect assessed in a CAM model. AuNPs functionalized with antiangiogenic peptides were used to block the angiogenic effect of CML-derived exosomes, assessed by evaluation of expression levels of key modulators involved in angiogenic pathways - VEGFA, VEGFR1 (also known as FLT1) and IL8.

Results

Exosomes isolated from K562 cells promot

SUBMITTER: Roma-Rodrigues C 

PROVIDER: S-EPMC6716571 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature