Sci-Hi-C: A single-cell Hi-C method for mapping 3D genome organization in large number of single cells.

ABSTRACT: The highly dynamic nature of chromosome conformation and three-dimensional (3D) genome organization leads to cell-to-cell variability in chromatin interactions within a cell population, even if the cells of the population appear to be functionally homogeneous. Hence, although Hi-C is a powerful tool for mapping 3D genome organization, this heterogeneity of chromosome higher order structure among individual cells limits the interpretive power of population based bulk Hi-C assays. Moreover, single-cell studies have the potential to enable the identification and characterization of rare cell populations or cell subtypes in a heterogeneous population. However, it may require surveying relatively large numbers of single cells to achieve statistically meaningful observations in single-cell studi

SUBMITTER: Ramani V 

PROVIDER: S-EPMC6949367 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature