Microarray analysis of miRNA expression profiles following whole body irradiation in a mouse model.
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ABSTRACT: Context
Accidental exposure to life-threatening radiation in a nuclear event is a major concern; there is an enormous need for identifying biomarkers for radiation biodosimetry to triage populations and treat critically exposed individuals.Objective
To identify dose-differentiating miRNA signatures from whole blood samples of whole body irradiated mice.Methods
Mice were whole body irradiated with X-rays (2 Gy-15 Gy); blood was collected at various time-points post-exposure; total RNA was isolated; miRNA microarrays were performed; miRNAs differentially expressed in irradiated vs. unirradiated controls were identified; feature extraction and classification models were applied to predict dose-differentiating miRNA signature.Results
We observed a time and dose responsive alteration in the expression levels of miRNAs. Maximum number of miRNAs were altered at 24-h and 48-h time-points post-irradiation. A 23-miRNA signature was identified using feature selection algorithms and classifier models. An inverse correlation in the expression level changes of miR-17 members, and their targets were observed in whole body irradiated mice and non-human primates.Conclusion
Whole blood-based miRNA expression signatures might be used for predicting radiation exposures in a mass casualty nuclear incident.
SUBMITTER: Aryankalayil MJ
PROVIDER: S-EPMC6982201 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
Microarray analysis of miRNA expression profiles following whole body irradiation in a mouse model.
Aryankalayil Molykutty J MJ Chopra Sunita S Chopra Sunita S Makinde Adeola A Eke Iris I Levin Joel J Shankavaram Uma U MacMillan Laurel L Vanpouille-Box Claire C Demaria Sandra S Coleman C Norman CN
Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals 20180619 7
<h4>Context</h4>Accidental exposure to life-threatening radiation in a nuclear event is a major concern; there is an enormous need for identifying biomarkers for radiation biodosimetry to triage populations and treat critically exposed individuals.<h4>Objective</h4>To identify dose-differentiating miRNA signatures from whole blood samples of whole body irradiated mice.<h4>Methods</h4>Mice were whole body irradiated with X-rays (2 Gy-15 Gy); blood was collected at various time-points post-exposur ...[more]
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