Microarray analysis of miRNA expression profiles following whole body irradiation in a mouse model.

ABSTRACT:

Context

Accidental exposure to life-threatening radiation in a nuclear event is a major concern; there is an enormous need for identifying biomarkers for radiation biodosimetry to triage populations and treat critically exposed individuals.

Objective

To identify dose-differentiating miRNA signatures from whole blood samples of whole body irradiated mice.

Methods

Mice were whole body irradiated with X-rays (2 Gy-15 Gy); blood was collected at various time-points post-exposure; total RNA was isolated; miRNA microarrays were performed; miRNAs differentially expressed in irradiated vs. unirradiated controls were identified; feature extraction and classification models were applied to predict dose-differentiating miRNA signature.

Results

We observed a time and dose

SUBMITTER: Aryankalayil MJ 

PROVIDER: S-EPMC6982201 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature