MicroRNA-181a regulates IFN-γ expression in effector CD8⁺ T cell differentiation.

ABSTRACT: CD8⁺ T cells are key players in immunity against intracellular infections and tumors. The main cytokine associated with these protective responses is interferon-γ (IFN-γ), whose production is known to be regulated at the transcriptional level during CD8⁺ T cell differentiation. Here we found that microRNAs constitute a posttranscriptional brake to IFN-γ expression by CD8⁺ T cells, since the genetic interference with the Dicer processing machinery resulted in the overproduction of IFN-γ by both thymic and peripheral CD8⁺ T cells. Using a gene reporter mouse for IFN-γ locus activity, we compared the microRNA repertoires associated with the presence or absence of IFN-γ expression. This allowed us to identify a set of candidates, including miR-181a a

SUBMITTER: Amado T 

PROVIDER: S-EPMC7007887 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature