Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae.

ABSTRACT:

Background

Sodium dodecyl sulfate (SDS) is one of the most widely used anionic alkyl sulfate surfactants. Toxicological information on SDS is accumulating, however, mechanisms of SDS toxicity regulation remain poorly understood. In this study, the relationship between the SDS-sensitive mutants and their intracellular ROS levels has been investigated.

Results

Through a genome-scale screen, we have identified 108 yeast single-gene deletion mutants that are sensitive to 0.03% SDS. These genes were predominantly related to the cellular processes of metabolism, cell cycle and DNA processing, cellular transport, transport facilities and transport routes, transcription and the protein with binding function or cofactor requirement (structural or catalytic). Measurement of the intrac

SUBMITTER: Cao C 

PROVIDER: S-EPMC7027087 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature