Rational flux-tuning of Halomonas bluephagenesis for co-production of bioplastic PHB and ectoine.

ABSTRACT: Ectoine, a compatible solute synthesized by many halophiles for hypersalinity resistance, has been successfully produced by metabolically engineered Halomonas bluephagenesis, which is a bioplastic poly(3-hydroxybutyrate) producer allowing open unsterile and continuous conditions. Here we report a de novo synthesis pathway for ectoine constructed into the chromosome of H. bluephagenesis utilizing two inducible systems, which serve to fine-tune the transcription levels of three clusters related to ectoine synthesis, including ectABC, lysC and asd based on a GFP-mediated transcriptional tuning approach. Combined with bypasses deletion, the resulting recombinant H. bluephagenesis TD-ADEL-58 is able to produce 28 g L^-1 ectoine during a 28 h fed-batch growth process. Co-production of

SUBMITTER: Ma H 

PROVIDER: S-EPMC7334215 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature