Dataset Information

Cation Exchange Protocols to Radiolabel Aqueous Stabilized ZnS, ZnSe, and CuFeS₂ Nanocrystals with ⁶⁴Cu for Dual Radio- and Photo-Thermal Therapy.

ABSTRACT: Here, cation exchange (CE) reactions are exploited to radiolabel ZnSe, ZnS, and CuFeS₂ metal chalcogenide nanocrystals (NCs) with ⁶⁴Cu. The CE protocol requires one simple step, to mix the water-soluble NCs with a ⁶⁴Cu solution, in the presence of vitamin C used to reduce Cu(II) to Cu(I). Given the quantitative cation replacement on the NCs, a high radiochemical yield, up to 99%, is reached. Also, provided that there is no free ⁶⁴Cu, no purification step is needed, making the protocol easily translatable to the clinic. A unique aspect of the approach is the achievement of an unprecedentedly high specific activity: by exploiting a volumetric CE, the strategy enables to concentrate a large dose of ⁶⁴Cu (18.5 MBq) in a small NC dose (0.18 µg), reaching a specific activity of 103 TBq g^-1. Finally, the characteristic dielectric resonance peak, still present for the radiolabeled ⁶⁴Cu:CuFeS₂ NCs after the partial-CE reaction, enables the generation of heat under clinical laser exposure (1 W cm^-2). The synergic toxicity of photo-ablation and ⁶⁴Cu ionization is here proven on glioblastoma and epidermoid carcinoma tumor cells, while no intrinsic cytotoxicity is seen from the NC dose employed for these dual experiments.

SUBMITTER: Avellini T

PROVIDER: S-EPMC7357593 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Cation Exchange Protocols to Radiolabel Aqueous Stabilized ZnS, ZnSe, and CuFeS2 Nanocrystals with 64Cu for Dual Radio- and Photo-Thermal Therapy.

Avellini Tommaso T Soni Nisarg N Silvestri Niccolò N Fiorito Sergio S De Donato Francesco F De Mei Claudia C Walther Martin M Cassani Marco M Ghosh Sandeep S Manna Liberato L Stephan Holger H Pellegrino Teresa T

Advanced functional materials 20200526 28

Here, cation exchange (CE) reactions are exploited to radiolabel ZnSe, ZnS, and CuFeS2 metal chalcogenide nanocrystals (NCs) with 64Cu. The CE protocol requires one simple step, to mix the water-soluble NCs with a 64Cu solution, in the presence of vitamin C used to reduce Cu(II) to Cu(I). Given the quantitative cation replacement on the NCs, a high radiochemical yield, up to 99%, is reached. Also, provided that there is no free 64Cu, no purification st ...[more]

PMID: 32684910

Similar Datasets

Project description:BackgroundDiacetyl-bis(N4-methylthiosemicarbazone), labeled with 64Cu (64Cu-ATSM) has been suggested as a promising tracer for imaging hypoxia. However, various controversial studies highlighted potential pitfalls that may disable its use as a selective hypoxic marker. They also highlighted that the results may be tumor location dependent. Here, we first analyzed uptake of Cu-ATSM and its less lipophilic counterpart Cu-Cl2 in the tumor over time in an orthotopic glioblastoma model. An in vitro study was also conducted to investigate the hypoxia-dependent copper uptake in tumor cells. We then further performed a comprehensive ex vivo study to compare 64Cu uptake to hypoxic markers, specific cellular reactions, and also transporter expression.MethodsμPET was performed 14 days (18F-FMISO), 15 days (64Cu-ATSM and 64Cu-Cl2), and 16 days (64Cu-ATSM and 64Cu-Cl2) after C6 cell inoculation. Thereafter, the brains were withdrawn for further autoradiography and immunohistochemistry. C6 cells were also grown in hypoxic workstation to analyze cellular uptake of Cu complexes in different oxygen levels.ResultsIn vivo results showed that Cu-ASTM and Cu-Cl2 accumulated in hypoxic areas of the tumors. Cu-ATSM also stained, to a lesser extent, non-hypoxic regions, such as regions of astrogliosis, with high expression of copper transporters and in particular DMT-1 and CTR1, and also characterized by the expression of elevated astrogliosis. In vitro results show that 64Cu-ATSM showed an increase in the uptake only in severe hypoxia at 0.5 and 0.2% of oxygen while for 64Cu-Cl2, the cell retention was significantly increased at 5% and 1% of oxygen with no significant rise at lower oxygen percentages.ConclusionIn the present study, we show that Cu-complexes undoubtedly accumulate in hypoxic areas of the tumors. This uptake may be the reflection of a direct dependency to a redox metabolism and also a reflection of hypoxic-induced overexpression of transporters. We also show that Cu-ATSM also stained non-hypoxic regions such as astrogliosis.

Dataset Information

Cation Exchange Protocols to Radiolabel Aqueous Stabilized ZnS, ZnSe, and CuFeS₂ Nanocrystals with ⁶⁴Cu for Dual Radio- and Photo-Thermal Therapy.

Publications

Cation Exchange Protocols to Radiolabel Aqueous Stabilized ZnS, ZnSe, and CuFeS<sub>2</sub> Nanocrystals with <sup>64</sup>Cu for Dual Radio- and Photo-Thermal Therapy.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets