High Throughput Co-culture Assays for the Investigation of Microbial Interactions.

ABSTRACT: The study of interactions between microorganisms has led to numerous discoveries, from novel antimicrobials to insights in microbial ecology. Many approaches used for the study of microbial interactions require specialized equipment and are expensive and time intensive. This paper presents a protocol for co-culture interaction assays that are inexpensive, scalable to large sample numbers, and easily adaptable to numerous experimental designs. Microorganisms are cultured together, with each well representing one pairwise combination of microorganisms. A test organism is cultured on one side of each well and first incubated in monoculture. Subsequently, target organisms are simultaneously inoculated onto the opposite side of each well using a 3D-printed inoculation stamp. After co-culture, t

SUBMITTER: Temkin MI 

PROVIDER: S-EPMC7380114 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature