Engineering Expression Cassette of pgdS for Efficient Production of Poly-γ-Glutamic Acids With Specific Molecular Weights in Bacillus licheniformis.

ABSTRACT: Poly-γ-glutamic acid (γ-PGA) is an emerging biopolymer with various applications and γ-PGAs with different molecular weights exhibit distinctive properties. However, studies on the controllable molecular weights of biopolymers are limited. The purpose of this study is to achieve production of γ-PGAs with a wide range of molecular weights through manipulating the expression of γ-PGA depolymerase (PgdS) in Bacillus licheniformis WX-02. Firstly, the expression and secretion of PgdS were regulated through engineering its expression elements (four promoters and eight signal peptides), which generated γ-PGAs with molecular weights ranging from 6.82 × 10⁴ to 1.78 × 10⁶ Da. Subsequently, through combination of promoters with signal peptides, the production of γ-PGAs wi

SUBMITTER: Wang D 

PROVIDER: S-EPMC7381323 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature