Determination of human identity from Anopheles stephensi mosquito blood meals using direct amplification and massively parallel sequencing.

ABSTRACT: DNA obtained from biological evidence can link individuals to a crime scene. DNA is typically obtained from body fluids deposited on various substrates such as fabric or common household objects. However, other unusual sources of human biological material can also be used to generate DNA profiles. Here we show that short tandem repeat (STR) DNA profiles can also be obtained from single source and mixtures of human DNA in the blood meals of Anopheles stephensi mosquitoes. Using direct amplification with the PowerPlex® Fusion 6C System, we have determined that full and partial profiles can be obtained by assessing degradation of DNA at various times post-feed up to 20-24 h post-blood meal. Moreover, we can assign donor identity through both STR profiles, as well as through single nucleotide

SUBMITTER: Gray SL 

PROVIDER: S-EPMC7487007 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature