Rapid desensitization of humanized mice with anti-human FcεRIα monoclonal antibodies.

ABSTRACT:

Background

Anaphylaxis is classically mediated by allergen cross-linking of IgE bound to the α chain of FcεRI, the mast cell/basophil high affinity IgE receptor. Allergen cross-linking of the IgE/FcεRI complex activates these cells, inducing release of disease-causing mediators, cytokines, and enzymes. We previously demonstrated that IgE-mediated anaphylaxis could be safely prevented in wild-type BALB/c mice by rapid desensitization with anti-mouse FcεRIα mAb.

Objective

This study sought to use humanized mice to extend these results to humans.

Methods

We actively immunized huFcεRIα/F709 mice, which express human (hu) instead of mouse FcεRIα and a mutant IL-4 receptor that lacks inhibitory function. We passively immunized huFcεRIα mice, as well as human cord blood-reco

SUBMITTER: Khodoun MV 

PROVIDER: S-EPMC7607676 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature