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Responsive fluorinated nanoemulsions for ¹⁹F magnetic resonance detection of cellular hypoxia.

ABSTRACT: We report two highly fluorinated Cu-based imaging agents, CuL1 and CuL2, for detecting cellular hypoxia as nanoemulsion formulations. Both complexes retained their initial quenched 19F MR signals due to paramagnetic Cu2+; however, both complexes displayed a large signal increase when the complex was reduced. DLS studies showed that the CuL1 nanoemulsion (NECuL1) had a hydrodiameter of approximately 100 nm and that it was stable for four weeks post-preparation. Hypoxic cells incubated with NECuL1 showed that 40% of the Cu2+ taken up was reduced in low oxygen environments.

SUBMITTER: Kadakia RT

PROVIDER: S-EPMC7688550 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Responsive fluorinated nanoemulsions for 19F magnetic resonance detection of cellular hypoxia.

Kadakia Rahul T RT Xie Da D Guo Hongyu H Bouley Bailey B Yu Meng M Que Emily L EL

Dalton transactions (Cambridge, England : 2003) 20201101 45

We report two highly fluorinated Cu-based imaging agents, CuL1 and CuL2, for detecting cellular hypoxia as nanoemulsion formulations. Both complexes retained their initial quenched 19F MR signals due to paramagnetic Cu2+; however, both complexes displayed a large signal increase when the complex was reduced. DLS studies showed that the CuL1 nanoemulsion (NECuL1) had a hydrodiameter of approximately 100 nm and that it was stable for four weeks post-preparation. Hypoxic cells incubated with NECuL1 ...[more]

PMID: 32692342

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