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Two-parameter single-molecule analysis for measurement of chromatin mobility.

ABSTRACT: This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).

SUBMITTER: Lerner J

PROVIDER: S-EPMC7757679 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Two-parameter single-molecule analysis for measurement of chromatin mobility.

Lerner Jonathan J Gómez-García Pablo Aurelio PA McCarthy Ryan L RL Liu Zhe Z Lakadamyali Melike M Zaret Kenneth S KS

STAR protocols 20201213 3

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and exec ...[more]

PMID: 33377115

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