Establishment and Application of a Dual TaqMan Real-Time PCR Method for Proteus Mirabilis and Proteus Vulgaris.

ABSTRACT: Proteus species are common opportunistic bacteria and foodborne pathogens. The proper detection of Proteus can effectively reduce the occurrence of food-borne public health events. Proteus mirabilis and Proteus vulgaris are the two most important pathogens in the Proteus genus. In this study, a dual TaqMan Real-Time PCR method was established to simultaneously detect and distinguish P. mirabilis and P. vulgaris in samples. The method exhibited good specificity, stability, and sensitivity. Specifically, the minimum detection concentrations of P. mirabilis and P. vulgaris in pure bacterial cultures were 6.08 × 10² colony forming units (CFU)/ml and 4.46 × 10² CFU/ml, respectively. Additionally, the minimum de

SUBMITTER: Yang R 

PROVIDER: S-EPMC7810114 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature