High throughput microRNAs sequencing profile of serum exosomes in women with and without polycystic ovarian syndrome.

ABSTRACT:

Background

Polycystic ovary syndrome (PCOS) is the most common type of endocrine disorder, affecting 5-11% of women of reproductive age worldwide. microRNAs (miRNAs) stably exist in circulating blood encapsulated in extracellular vesicles such as exosomes; therefore, serum miRNAs have the potential to serve as novel PCOS biomarkers.

Methods

To identify miRNA biomarkers that are associated with PCOS, we performed a comprehensive sequence-based characterization of the PCOS serum miRNA landscape. The serum exosomes were successfully isolated and characterized in a variety of ways. Next, sequence-based analysis was performed on serum exosomes to screen the differentially expressed miRNAs in women with and without PCOS.

Results

The sequence data revealed that the levels of

SUBMITTER: Zhang F 

PROVIDER: S-EPMC7958896 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature