To Ki or Not to Ki: Re-Evaluating the Use and Potentials of Ki-67 for T Cell Analysis.

ABSTRACT: This study discusses substantive advances in T cell proliferation analysis, with the aim to provoke a re-evaluation of the generally-held view that Ki-67 is a reliable proliferation marker per se, and to offer a more sensitive and effective method for T cell cycle analysis, with informative examples in mouse and human settings. We summarize recent experimental work from our labs showing that, by Ki-67/DNA dual staining and refined flow cytometric methods, we were able to identify T cells in the S-G₂/M phases of the cell-cycle in the peripheral blood (collectively termed "T Double S" for T cells in S-phase in Sanguine: in short "T_DS" cells). Without our refinement, such cells may be excluded from conventional lymphocyte analyses. Specifically, we analyzed

SUBMITTER: Di Rosa F 

PROVIDER: S-EPMC8062736 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature