Robust, coherent, and synchronized circadian clock-controlled oscillations along Anabaena filaments.

ABSTRACT: Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in Anabaena sp. PCC 7120, a multicellular filamentous cyanobacterium. We found significant synchronization and spatial coherence along filaments, clock coupling due to cell-cell communication, and gating of the cell cycle. Furthermore, we observed low-amplitude circadian oscillatory transcription of kai genes encoding the post-transcriptional core oscillatory circuit and high-amplitude oscillations of rpaA coding for the master regulator transducing the core clock output. Transcriptional oscillations of rpaA suggest an additional level of regulation. A stochastic one-d

SUBMITTER: Arbel-Goren R 

PROVIDER: S-EPMC8064755 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature