Linchpins empower promiscuous electrophiles to enable site-selective modification of histidine and aspartic acid in proteins.

ABSTRACT: The conservation of chemoselectivity becomes invalid for multiple electrophilic warheads during protein bioconjugation. Consequently, it leads to unpredictable heterogeneous labeling of proteins. Here, we report that a linchpin can create a unique chemical space to enable site-selectivity for histidine and aspartic acid modifications overcoming the pre-requisite of chemoselectivity.

SUBMITTER: Rawale DG 

PROVIDER: S-EPMC8133000 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature