Evaluation of sample treatments in a safe and straightforward procedure for the detection of SARS-CoV-2 in saliva.

ABSTRACT:

Objectives

To evaluate four sample treatments in a safe and straightforward procedure to detect SARS-CoV-2 in saliva.

Methods

Four sample treatments were evaluated in a 3-step procedure to detect SARS-CoV-2 in saliva: 1) heating at 95 °C for 5 min for sample inactivation; 2) sample treatment; 3) analysis by reverse-transcription loop-mediated isothermal amplification (LAMP). Saliva samples used were from infected individuals or were spiked with known quantities of viral particles.

Results

Three treatments had a limit of detection (LOD) of 500.000 viral particles per ml of saliva and could be used to detect individuals with potential to transmit the disease. The treatment of phosphate buffer, dithiothreitol, ethylenediaminetetraacetic acid and proteinase K, with an add

SUBMITTER: Rubio CP 

PROVIDER: S-EPMC8142814 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature