Combined single-molecule fluorescence in situ hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve.
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ABSTRACT:
SUBMITTER: Li X
PROVIDER: S-EPMC8185307 | biostudies-literature | 2021 Jun
REPOSITORIES: biostudies-literature
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Combined single-molecule fluorescence <i>in situ</i> hybridization and immunohistochemistry analysis in intact murine dorsal root ganglia and sciatic nerve.
Li Xinbei X Eadara Sreenivas S Jeon Sangmin S Liu Yan Y Muwanga Gabriella G Qu Lintao L Caterina Michael J MJ Meffert Mollie K MK
STAR protocols 20210603 2
Single-molecule fluorescence <i>in situ</i> hybridization (smFISH) allows spatial mapping of gene expression. This protocol presents advances in smFISH fidelity and flexibility in intact murine sensory nervous system tissue. An approach using RNAscope probes allows multiplexing, enhanced target specificity, and immunohistochemistry compatibility. Computational strategies increase quantification accuracy of mRNA puncta with a point spread function for clustered transcripts in the dorsal root gang ...[more]
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