Evaluation of the effect of donor weight on adipose stromal/stem cell characteristics by using weight-discordant monozygotic twin pairs.

ABSTRACT:

Background

Adipose stromal/stem cells (ASCs) are promising candidates for future clinical applications. ASCs have regenerative capacity, low immunogenicity, and immunomodulatory ability. The success of future cell-based therapies depends on the appropriate selection of donors. Several factors, including age, sex, and body mass index (BMI), may influence ASC characteristics. Our aim was to investigate the effect of acquired weight on ASC characteristics under the same genetic background using ASCs derived from monozygotic (MZ) twin pairs.

Methods

ASCs were isolated from subcutaneous adipose tissue from five weight-discordant (WD, within-pair difference in BMI > 3 kg/m²) MZ twin pairs, with measured BMI and metabolic status. The ASC immunophenotype, proliferation an

SUBMITTER: Juntunen M 

PROVIDER: S-EPMC8474937 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature