Dataset Information

A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker.

ABSTRACT: Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a "turn-on" bioluminescent readout of a C. difficile-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was "caged" with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was released and NanoLuc activity was restored, providing a visible readout. The bioluminescent sensor detected PPEP-1 concentrations as low as 10 nM. Sensor uncaging was achieved within minutes, and signal was captured using a digital camera. Importantly, the sensor was also functional at ambient temperature and compatible with fecal material, suggesting that it can be readily deployed in a variety of settings.

SUBMITTER: Ng KK

PROVIDER: S-EPMC8624784 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker.

Ng Kevin K KK Reinert Zachary E ZE Corver Jeroen J Resurreccion Danica D Hensbergen Paul J PJ Prescher Jennifer A JA

Sensors (Basel, Switzerland) 20211111 22

Current assays for Clostridioides difficile in nonhospital settings are outsourced and time-intensive, resulting in both delayed diagnosis and quarantining of infected individuals. We designed a more rapid point-of-care assay featuring a "turn-on" bioluminescent readout of a C. difficile-specific protease, PPEP-1. NanoLuc, a bright and stable luciferase, was "caged" with a PPEP-1-responsive peptide tail that inhibited luminescence. Upon proteolytic cleavage, the peptide was release ...[more]

PMID: 34833562

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Dataset Information

A Bioluminescent Sensor for Rapid Detection of PPEP-1, a Clostridioides difficile Biomarker.

Publications

A Bioluminescent Sensor for Rapid Detection of PPEP-1, a <i>Clostridioides difficile</i> Biomarker.

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