Chloroplast genome sequencing based on genome skimming for identification of Eriobotryae Folium.

ABSTRACT:

Background

Whole chloroplast genome (cpDNA) sequence is becoming widely used in the phylogenetic studies of plant and species identification, but in most cases the cpDNA were acquired from silica gel dried fresh leaves. So far few reports have been available to describe cpDNA acquisition from crude drugs derived from plant materials, the DNA of which usually was seriously damaged during their processing. In this study, we retrieved cpDNA from the commonly used crude drug Eriobotryae Folium (Pipaye in Chinese, which is the dried leaves of Eriobotrya japonica, PPY) using genome skimming technique.

Results

We successfully recovered cpDNA sequences and rDNA sequences from the crude drug PPY, and bioinformatics analysis showed a high overall consistency between the cpDNA obtained

SUBMITTER: Li F 

PROVIDER: S-EPMC8666020 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature