Characterizing and engineering promoters for metabolic engineering of Ogataea polymorpha.

ABSTRACT: Bio-manufacturing via microbial cell factory requires large promoter library for fine-tuned metabolic engineering. Ogataea polymorpha, one of the methylotrophic yeasts, possesses advantages in broad substrate spectrum, thermal-tolerance, and capacity to achieve high-density fermentation. However, a limited number of available promoters hinders the engineering of O. polymorpha for bio-productions. Here, we systematically characterized native promoters in O. polymorpha by both GFP fluorescence and fatty alcohol biosynthesis. Ten constitutive promoters (P _PDH , P _PYK , P _FBA , P _PGM , P _GLK , P _TRI , P _GPI , P _ADH1 , P _TEF

SUBMITTER: Yan C 

PROVIDER: S-EPMC8685918 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature