A proteolytic nanobiocatalyst with built-in disulphide reducing properties.

ABSTRACT: We report a method to equip proteolytic nanobiocatalysts with intrinsic disulphide bond reducing properties. After immobilisation onto silica particles, selected protease enzymes are partially shielded in a nanometre-thick mercaptosilica layer acting not only as a protective system but also as a substrate reducing agent. The biocatalysts produced efficiently perform simultaneous disulphide bond reduction and protein digestion. Besides a significant simplification of the proteolysis process, this strategy allows for a drastic increase of the enzyme stability.

SUBMITTER: Briand ML 

PROVIDER: S-EPMC8693372 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature