Quantifying phosphorylation dynamics in primary neuronal cultures using LC-MS/MS.
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ABSTRACT:
SUBMITTER: Desch K
PROVIDER: S-EPMC8715330 | biostudies-literature | 2022 Mar
REPOSITORIES: biostudies-literature
Publications
Quantifying phosphorylation dynamics in primary neuronal cultures using LC-MS/MS.
Desch Kristina K Schuman Erin M EM Langer Julian D JD
STAR protocols 20211222 1
Cellular processes require tight and coordinated control of protein abundance, localization, and activity. One of the core mechanisms to achieve specific regulation of proteins is protein phosphorylation. Here we present a workflow to monitor protein abundance and phosphorylation in primary cultured neurons using liquid chromatography-coupled mass spectrometry. Our protocol provides a detailed guide on all steps for detection and label-free-quantification of phosphorylated and unmodified protein ...[more]
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