Hyperosmolar Ionic Solutions Modulate Inflammatory Phenotype and sGAG Loss in a Cartilage Explant Model.

ABSTRACT:

Objective

The objective of this study was to compare the effects of hyperosmolar sodium (Na⁺), lithium (Li⁺) and potassium (K⁺) on catabolic and inflammatory osteoarthritis (OA) markers and sulfated glycosaminoglycan (sGAG) loss in TNF-α-stimulated cartilage explants.

Methods

Explants from bovine stifle joints were stimulated with TNF-α for 1 day to induce cartilage degradation followed by supplementation with 50 mM potassium chloride (KCl), 50 mM lithium chloride (LiCl), 50 mM sodium chloride (NaCl), or 100 nM dexamethasone for an additional 6 days. We assessed the effect of TNF-α stimulation and hyperosmolar ionic treatment on sGAG loss and expression of OA-associated proteins: ADAMTS-5, COX-2, MMP-1, MMP-13, and VEGF.

Results

TNF-α

SUBMITTER: Arabiyat AS 

PROVIDER: S-EPMC8804856 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature