Ontology highlight
ABSTRACT:
SUBMITTER: Gurzeler LA
PROVIDER: S-EPMC8815625 | biostudies-literature | 2022 Jan
REPOSITORIES: biostudies-literature
Gurzeler Lukas-Adrian LA Ziegelmüller Jana J Mühlemann Oliver O Karousis Evangelos D ED
RNA biology 20211229 1
Protein synthesis is a central process in gene expression and the development of efficient <i>in vitro</i> translation systems has been the focus of scientific efforts for decades. The production of translation-competent lysates originating from human cells or tissues remains challenging, mainly due to the variability of cell lysis conditions. Here we present a robust and fast method based on dual centrifugation that allows for detergent-free cell lysis under controlled mechanical forces. We opt ...[more]