Dataset Information

Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay.

ABSTRACT: We introduce a simple-to-use manual roller pump (MRP)-driven and valve-free microfluidic system for sequential solution exchange, followed by a bioassay to detect protein. The polydimethylsiloxane (PDMS)/glass-based disposable device comprises a reaction chamber, multiple micro-flow channels (μFCs), and air vents. The practical solution exchange was realized by sequential injection and withdrawal of several solutions into and from the reaction chamber through constricted μFCs by utilizing changing air pressure of an MRP when a small cylindrical roller was pressed and rolled over a soft silicone tube using a finger. Furthermore, we investigated the effect of surface hydrophobicity on solution exchange. A sandwich fluorescence-based immunoassay to detect human interleukin 2 (IL-2) was performed using this simple microfluidic scheme to demonstrate its suitability for analytical bioassays. The system allowed quick IL-2 detection in 20 min in a pre-functionalized device with a detection limit of 80 pg mL^-1 and a range of 125 pg mL^-1 to 2.0 ng mL^-1. We have thus developed a microfluidic scheme that non-experts can efficiently perform and that can be the fundamental module for low-cost bioassays necessary for emergencies and situations where resources are constrained.

SUBMITTER: Biswas GC

PROVIDER: S-EPMC8979114 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay.

Biswas Gokul Chandra GC Suzuki Hiroaki H

RSC advances 20220121 5

We introduce a simple-to-use manual roller pump (MRP)-driven and valve-free microfluidic system for sequential solution exchange, followed by a bioassay to detect protein. The polydimethylsiloxane (PDMS)/glass-based disposable device comprises a reaction chamber, multiple micro-flow channels (μFCs), and air vents. The practical solution exchange was realized by sequential injection and withdrawal of several solutions into and from the reaction chamber through constricted μFCs by utilizing changi ...[more]

PMID: 35425303

Similar Datasets

Project description:ObjectivesTo assess the effectiveness of injectable tissue pulmonary valve compared with standard pulmonary valve in patients requiring pulmonary valve replacement surgery.DesignA multicentre, single-blind, parallel two-group randomised controlled trial. Participants were blind to their allocation. Follow-up continued for 6 months. Randomised allocations were generated by a computer using block randomisation, stratified by centre.SettingTwo National Health Service secondary care centres in the UK.ParticipantsPeople aged 12-80 years requiring pulmonary valve replacement.InterventionsParticipants were randomly allocated (1:1 ratio) to injectable pulmonary valve replacement (IPVR) without cardiopulmonary bypass (CPB) or standard pulmonary valve replacement (SPVR) with CPB.Primary and secondary outcome measuresThe primary outcome was chest drainage volume over the first 24 hours after surgery. Secondary outcomes included in-hospital clinical outcomes; valve and heart function 6 months postsurgery and health-related quality of life 6 weeks and 6 months postsurgery.ResultsNineteen participants agreed to take part. Eleven were allocated to IPVR and eight to SPVR. The trial was stopped before the target sample size of 60 participants was reached due to challenges in recruitment. The primary analysis includes all randomised participants; there were no withdrawals. Chest drain volume 24 hours after surgery was on average 277.6 mL lower with IPVR (IPVR mean 340.0 mL; SPVR mean 633.8 mL; mean difference, -277.6; 95% CI, -484.0 to -71.2; p=0.005). There were no statistically significant differences in time to readiness for extubation (p=0.476), time to fitness for discharge (p=0.577) and time to first discharge from the intensive care unit (p=0.209). Six participants with IPVR required CPB. Safety profiles and quality of life scores were similar.ConclusionsIPVR reduced chest drain volume despite >50% of participants requiring CPB. There was no evidence of any other benefit of IPVR.Trial registration numberISRCTN23538073.

Dataset Information

Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay.

Publications

Simple manual roller pump-driven valve-free microfluidic solution exchange system for urgent bioassay.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets