Phenotype-Based Isolation of Antigen-Specific CD4⁺ T Cells in Autoimmunity: A Study of Celiac Disease.

ABSTRACT: The pathogenic immune response in celiac disease (CeD) is orchestrated by phenotypically distinct CD4⁺ T cells that recognize gluten epitopes in the context of disease-associated HLA-DQ allotypes. Cells with the same distinct phenotype, but with elusive specificities, are increased across multiple autoimmune conditions. Here, whether sorting of T cells based on their distinct phenotype (Tphe cells) yields gluten-reactive cells in CeD is tested. The method's efficiency is benchmarked by parallel isolation of gluten-reactive T cells (Ttet cells), using HLA-DQ:gluten peptide tetramers. From gut biopsies of 12 untreated HLA-DQ2.5⁺ CeD patients, Ttet⁺ /Tphe⁺ , Ttet^- /Tphe⁺ , and Ttet^- /Tphe^- cells are sorted

SUBMITTER: Christophersen A 

PROVIDER: S-EPMC8981484 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature