In vitro production of desired sex ovine embryos modulating polarity of oocytes for sex-specific sperm binding during fertilization.

ABSTRACT: The present study aimed to modulate the oxidative status-mediated polarity of the oocytes for sex-specific sperm fertilization to generate desired sex embryos. In vitro embryos were produced at different oxidative status, varying O₂ concentrations, and without/with L-carnitine in maturation and culture media. The majority of the embryos produced at high oxidative stress were males whereas; low oxidative status favoured female embryos production. Low O₂ doubled the proportion of female embryos (10.59 vs 21.95%); however, L-carnitine supplementation in media increased approximately seven-folds of the female embryos (12.26 vs. 77.62%) production. Oocytes matured at high oxidative status were in the repolarized state favouring positively charged Y sperm fertilization to p

SUBMITTER: G RK 

PROVIDER: S-EPMC8991187 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature