L-Type Ca_v1.3 Calcium Channels Are Required for Beta-Adrenergic Triggered Automaticity in Dormant Mouse Sinoatrial Pacemaker Cells.

ABSTRACT:

Background

Sinoatrial node cells (SANC) automaticity is generated by functional association between the activity of plasmalemmal ion channels and local diastolic intracellular Ca²⁺ release (LCR) from ryanodine receptors. Strikingly, most isolated SANC exhibit a "dormant" state, whereas only a fraction shows regular firing as observed in intact SAN. Recent studies showed that β-adrenergic stimulation can initiate spontaneous firing in dormant SANC, though this mechanism is not entirely understood.

Methods

To investigate the role of L-type Ca_v1.3 Ca²⁺ channels in the adrenergic regulation of automaticity in dormant SANC, we used a knock-in mouse strain in which the sensitivity of L-type Ca_v1.2 α1 subunits to dihydropyridines (DHPs) w

SUBMITTER: Louradour J 

PROVIDER: S-EPMC8997967 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature