Ceramide changes in abdominal subcutaneous and visceral adipose tissue among diabetic and nondiabetic patients.

ABSTRACT:

Background

This study profiles ceramides extracted from visceral and subcutaneous adipose tissue of human subjects by liquid chromatography-mass spectrometry to determine a correlation with status of diabetes and gender.

Methods

Samples of visceral and abdominal wall subcutaneous adipose tissue (n = 36 and n = 31, respectively) were taken during laparoscopic surgery from 36 patients (14 nondiabetic, 22 diabetic and prediabetic) undergoing bariatric surgery with a body mass index (BMI) >35 kg/m² with ≥1 existing comorbidity or BMI ≥40 kg/m² . Sphingolipids were extracted and analyzed using liquid chromatography-mass spectrometry.

Results

After logarithm 2 conversion, paired analysis of visceral to subcutaneous tissue showed differential accumulati

SUBMITTER: Brusatori M 

PROVIDER: S-EPMC9060146 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature