In vivo tissue optical clearing assisted through-skull targeted photothrombotic ischemic stroke model in mice.

ABSTRACT:

Significance

Photothrombotic stroke is an important and widely used model for ischemic stroke research. However, the significant scattering of the skull during the procedure limits the light's ability to penetrate and focus on its target. Targeted photothrombosis uses surgery-based skull windows to obtain optical access to the brain, but it renders the brain's environment unnatural even before a stroke is established.

Aim

To establish a targeted, controllable ischemic stroke model in mice through an intact skull.

Approach

The in vivo skull optical clearing technique provides a craniotomy-free "optical window" that allows light to penetrate. Alongside the local photodynamic effect, we have established targeted photothrombosis without skull removal, effectively controll

SUBMITTER: Hu Z 

PROVIDER: S-EPMC9174889 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature