Dataset Information

Jaw1/LRMP increases Ca²⁺ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype.

ABSTRACT: Ca²⁺ influx upon G protein-coupled receptor (GPCR) stimulation is observed as a cytosolic Ca²⁺ concentration oscillation crucial to initiating downstream responses including cell proliferation, differentiation, and cell-cell communication. Although Jaw1 is known to interact with inositol 1,4,5-triphosphate receptor (ITPRs), Ca²⁺ channels on the endoplasmic reticulum, the function of Jaw1 in the Ca²⁺ dynamics with physiological stimulation remains unclear. In this study, using inducible Jaw1-expressing HEK293 cells, we showed that Jaw1 increases Ca²⁺ influx by GPCR stimulation via changing the Ca²⁺ influx oscillation pattern. Furthermore, we showed that Jaw1 increases the Ca²⁺ release activity of all ITPR subtypes in a subtly different manner. It is well known that the Ca²⁺ influx oscillation pattern varies from cell type to cell type, therefore these findings provide an insight into the relationship between the heterogeneous Ca²⁺ dynamics and the specific ITPR and Jaw1 expression patterns.

SUBMITTER: Okumura W

PROVIDER: S-EPMC9177832 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature

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Jaw1/LRMP increases Ca2+ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype.

Okumura Wataru W Kozono Takuma T Sato Hiroyuki H Matsui Hitomi H Takagi Tsubasa T Tonozuka Takashi T Nishikawa Atsushi A

Scientific reports 20220608 1

Ca2+ influx upon G protein-coupled receptor (GPCR) stimulation is observed as a cytosolic Ca2+ concentration oscillation crucial to initiating downstream responses including cell proliferation, differentiation, and cell-cell communication. Although Jaw1 is known to interact with inositol 1,4,5-triphosphate receptor (ITPRs), Ca2+ channels on the endoplasmic reticulum, the function of Jaw1 in the Ca2+ dynamics with physiological stimulation remains uncle ...[more]

PMID: 35676525

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Dataset Information

Jaw1/LRMP increases Ca²⁺ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype.

Publications

Jaw1/LRMP increases Ca<sup>2+</sup> influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype.

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