Jaw1/LRMP increases Ca²⁺ influx upon GPCR stimulation with heterogeneous effect on the activity of each ITPR subtype.

ABSTRACT: Ca²⁺ influx upon G protein-coupled receptor (GPCR) stimulation is observed as a cytosolic Ca²⁺ concentration oscillation crucial to initiating downstream responses including cell proliferation, differentiation, and cell-cell communication. Although Jaw1 is known to interact with inositol 1,4,5-triphosphate receptor (ITPRs), Ca²⁺ channels on the endoplasmic reticulum, the function of Jaw1 in the Ca²⁺ dynamics with physiological stimulation remains unclear. In this study, using inducible Jaw1-expressing HEK293 cells, we showed that Jaw1 increases Ca²⁺ influx by GPCR stimulation via changing the Ca²⁺ influx oscillation pattern. Furthermore, we showed that Jaw1 increases the Ca²⁺ release activity of all ITPR subtypes

SUBMITTER: Okumura W 

PROVIDER: S-EPMC9177832 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature