Dataset Information

Serum Soluble Mediator Profiles and Networks During Acute Infection With Distinct DENV Serotypes.

ABSTRACT: A panoramic analysis of chemokines, pro-inflammatory/regulatory cytokines, and growth factors was performed in serum samples from patients with acute DENV infection (n=317) by a high-throughput microbeads array. Most soluble mediators analyzed were increased in DENV patients regardless of the DENV serotype. The substantial increase (≥10-fold) of CXCL10, IL-6, and IFN-γ, and decreased levels of PDGF (<0.4-fold) was universally identified in all DENV serotypes. Of note, increased levels of CXCL8, CCL4, and IL-12 (≥3-9-fold) were selectively observed in DENV2 as compared to DENV1 and DENV4. Heatmap and biomarker signatures further illustrated the massive release of soluble mediators observed in DENV patients, confirming the marked increase of several soluble mediators in DENV2. Integrative correlation matrices and networks showed that DENV infection exhibited higher connectivity among soluble mediators. Of note, DENV2 displayed a more complex network, with higher connectivity involving a higher number of soluble mediators. The timeline kinetics (Day 0-1, D2, D3, D4-6) analysis additionally demonstrated differences among DENV serotypes. While DENV1 triggers a progressive increase of soluble mediators towards D3 and with a decline at D4-6, DENV2 and DENV4 develop with a progressive increase towards D4-6 with an early plateau observed in DENV4. Overall, our results provided a comprehensive overview of the immune response elicited by DENV infection, revealing that infection with distinct DENV serotypes causes distinct profiles, rhythms, and dynamic network connectivity of soluble mediators. Altogether, these findings may provide novel insights to understand the pathogenesis of acute infection with distinct DENV serotypes.

SUBMITTER: Coutinho-da-Silva MS

PROVIDER: S-EPMC9193801 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Serum Soluble Mediator Profiles and Networks During Acute Infection With Distinct DENV Serotypes.

Coutinho-da-Silva Mikelly Santos MS Sucupira Pedro Henrique Ferreira PHF Bicalho Kelly Alves KA Campi-Azevedo Ana Carolina AC Brito-de-Sousa Joaquim Pedro JP Peruhype-Magalhães Vanessa V Rios Maria M Teixeira-Carvalho Andréa A Coelho-Dos-Reis Jordana Grazziela Alves JGA Antonelli Lis Ribeiro do Valle LRDV de Rezende Vitor Bortolo VB de Melo Fernanda Ludolf Ribeiro FLR Garcia Cristiana Couto CC Silva-Andrade Jesuanne Carla JC da Costa-Rocha Ismael Artur IA Bastos Michele de Souza MS da Rocha Lucia Alves LA Silva Valderjane Aprigio VA Ferreira Ewerton da Silva EDS Marinho Eveny Perlize Melo EPM Costa Allyson Guimarães AG Gomes Matheus de Souza MS Amaral Laurence Rodrigues LR Furtado Erilene Cristina da Silva ECDS da Silva Eliana Vieira Pinto EVP Ramos Bruna Alves BA Dos Santos Éder Barros ÉB Freitas Maria Nazaré Oliveira MNO Vasconcelos Pedro Fernando da Costa PFDC Martins-Filho Olindo Assis OA Araújo Márcio Sobreira Silva MSS Ferreira Milene Silveira MS Martins Livia Carício LC

Frontiers in immunology 20220531

A panoramic analysis of chemokines, pro-inflammatory/regulatory cytokines, and growth factors was performed in serum samples from patients with acute DENV infection (n=317) by a high-throughput microbeads array. Most soluble mediators analyzed were increased in DENV patients regardless of the DENV serotype. The substantial increase (≥10-fold) of CXCL10, IL-6, and IFN-γ, and decreased levels of PDGF (<0.4-fold) was universally identified in all DENV serotypes. Of note, increased levels of CXCL8, ...[more]

PMID: 35711447

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Project description:The COVID-19 pandemic has caused a severe global health and economic crisis, with significant consequences for human mortality and morbidity. Therefore, there is an urgent need for more studies on the immune response to SARS-CoV-2 infection, both to enhance its effectiveness and prevent its deleterious effects. This study presents the chronology of antibodies during six months after infection in hospitalized patients and the kinetics of serum soluble mediators of the cellular response triggered by SARS-CoV-2. Samples and clinical data from 330 patients hospitalized at the Hospital da Baleia in Belo Horizonte, Brazil, who were suspected of having COVID-19, were collected at the time of hospitalization and during 6 months after infection. The immune response was analyzed by enzyme-linked immunosorbent assay (ELISA) and flow cytometry. There was a significant difference in IgM specific antibody titers from the 7th to 60th days after infection between COVID-19 negative and positive patients. Soon after 60 days after infection, antibody levels started to reduce, becoming similar to the antibody levels of the COVID-19 negative patients. IgG specific antibodies started to be detectable after 9 days of infection and antibody levels were comparatively higher in positive patients as soon as after 7 days. Furthermore, IgG levels remained higher in these patients during the complete period of 180 days after infection. The study observed similar antibody profiles between different patient groups. The soluble systemic biomarkers evaluated showed a decrease during the six months after hospitalization, except for CCL11, CXCL8, CCL3, CCL4, CCL5, IL-6, IFN-g, IL-17, IL-5, FGF-basic, PDGF, VEGF, G-CSF, and GM-CSF. The results indicate that IgM antibodies are more prominent in the early stages of infection, while IgG antibodies persist for a longer period. Additionally, the study identified that patients with COVID-19 have elevated levels of biomarkers after symptom onset, which decrease over time.

Dataset Information

Serum Soluble Mediator Profiles and Networks During Acute Infection With Distinct DENV Serotypes.

Publications

Serum Soluble Mediator Profiles and Networks During Acute Infection With Distinct DENV Serotypes.

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