Temporal and Spatial Changes in the Microbiome Following Pediatric Severe Traumatic Brain Injury.

ABSTRACT:

Objectives

The microbiome may be affected by trauma and critical illness. Many studies of the microbiome in critical illness are restricted to a single body site or time point and confounded by preexisting conditions. We report temporal and spatial alterations in the microbiome of previously healthy children with severe traumatic brain injury (TBI).

Design

We collected oral, rectal, and skin swabs within 72 hours of admission and then twice weekly until ICU discharge. Samples were analyzed by 16S rRNA gene amplicon sequencing. Children undergoing elective outpatient surgery served as controls. Alpha and beta diversity comparisons were performed with Phyloseq, and differentially abundant taxa were predicted using Analysis of Composition of Microbiomes.

Setting

Five qua

SUBMITTER: Rogers MB 

PROVIDER: S-EPMC9203870 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature