Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis.

ABSTRACT: The murine excisional wound model has been used extensively to study each of the sequentially overlapping phases of wound healing: inflammation, proliferation and remodeling. Murine wounds have a histologically well-defined and easily recognizable wound bed over which these different phases of the healing process are measurable. Within the field, it is common to use an arbitrarily defined "middle" of the wound for histological analyses. However, wounds are a three-dimensional entity and often not histologically symmetrical, supporting the need for a well-defined and robust method of quantification to detect morphometric defects with a small effect size. In this protocol, we describe the procedure for creating bilateral, full-thickness excisional wounds in mice as well as a detailed instruc

SUBMITTER: Rhea L 

PROVIDER: S-EPMC9280391 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature