Dataset Information

Complex B₁⁺ mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

ABSTRACT:

Purpose

To present a new complex-valued B₁⁺ mapping method for electrical properties tomography using Carr-Purcell spin echoes.

Methods

A Carr-Purcell (CP) echo train generates pronounced flip-angle dependent oscillations that can be used to estimate the magnitude of B₁⁺ . To this end, a dictionary is used that takes into account the slice profile as well as T₂ relaxation along the echo train. For validation, the retrieved B₁⁺ map is compared with the actual flip angle imaging (AFI) method in a phantom (79 ε₀ , 0.34 S/m). Moreover, the phase of the first echo reflects the transceive phase. Overall, the CP echo train yields an estimate of the complex-valued B₁⁺ , allowing electrical properties tomography with both permittivity and conductivity. The presented method is evaluated in phantom scans as well as for in vivo brain at 3 T.

Results

In the phantom, the obtained magnitude B₁⁺ maps retrieved from the CP echo train and the AFI method show excellent agreement, and both the reconstructed estimated permittivity (79 ± 3) ε₀ and conductivity (0.35 ± 0.04) S/m values are in accordance with expectations. In the brain, the obtained electrical properties are also close to expectations. In addition to the retrieved complex B₁⁺ information, the decay of the CP echo trains also yields an estimate for T₂ .

Conclusion

The CP sequence can be used to simultaneously provide both B₁⁺ magnitude and phase estimations, and therefore allows for full reconstruction of the electrical properties.

SUBMITTER: Iyyakkunnel S

PROVIDER: S-EPMC9298742 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Publications

Complex B<sub>1</sub><sup>+</sup> mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

Iyyakkunnel Santhosh S Weigel Matthias M Ganter Carl C Bieri Oliver O

Magnetic resonance in medicine 20211109 3

<h4>Purpose</h4>To present a new complex-valued B<sub>1</sub><sup>+</sup> mapping method for electrical properties tomography using Carr-Purcell spin echoes.<h4>Methods</h4>A Carr-Purcell (CP) echo train generates pronounced flip-angle dependent oscillations that can be used to estimate the magnitude of B<sub>1</sub><sup>+</sup> . To this end, a dictionary is used that takes into account the slice profile as well as T<sub>2</sub> relaxation along the echo train. For validation, the retrieved B<s ...[more]

PMID: 34752636

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Project description:To study microsecond processes by relaxation dispersion NMR spectroscopy, low power deposition and short pulses are crucial and encourage the development of experiments that employ (1)H Carr-Purcell-Meiboom-Gill (CPMG) pulse trains. Herein, a method is described for the comprehensive study of microsecond to millisecond time scale dynamics of methyl groups in proteins, exploiting their high abundance and favorable relaxation properties. In our approach, protein samples are produced using [(1)H, (13)C]-d-glucose in ∼100% D(2)O, which yields CHD(2) methyl groups for alanine, valine, threonine, isoleucine, leucine, and methionine residues with high abundance, in an otherwise largely deuterated background. Methyl groups in such samples can be sequence-specifically assigned to near completion, using (13)C TOCSY NMR spectroscopy, as was recently demonstrated (Otten, R.; et al. J. Am. Chem. Soc. 2010, 132, 2952-2960). In this Article, NMR pulse schemes are presented to measure (1)H CPMG relaxation dispersion profiles for CHD(2) methyl groups, in a vein similar to that of backbone relaxation experiments. Because of the high deuteration level of methyl-bearing side chains, artifacts arising from proton scalar coupling during the CPMG pulse train are negligible, with the exception of Ile-δ1 and Thr-γ2 methyl groups, and a pulse scheme is described to remove the artifacts for those residues. Strong (13)C scalar coupling effects, observed for several leucine residues, are removed by alternative biochemical and NMR approaches. The methodology is applied to the transcriptional activator NtrC(r), for which an inactive/active state transition was previously measured and the motions in the microsecond time range were estimated through a combination of backbone (15)N CPMG dispersion NMR spectroscopy and a collection of experiments to determine the exchange-free component to the transverse relaxation rate. Exchange contributions to the (1)H line width were detected for 21 methyl groups, and these probes were found to collectively report on a local structural rearrangement around the phosphorylation site, with a rate constant of (15.5 ± 0.5) × 10(3) per second (i.e., τ(ex) = 64.7 ± 1.9 μs). The affected methyl groups indicate that, already before phosphorylation, a substantial, transient rearrangement takes place between helices 3 and 4 and strands 4 and 5. This conformational equilibrium allows the protein to gain access to the active, signaling state in the absence of covalent modification through a shift in a pre-existing dynamic equilibrium. Moreover, the conformational switching maps exactly to the regions that differ between the solution NMR structures of the fully inactive and active states. These results demonstrate that a cost-effective and quantitative study of protein methyl group dynamics by (1)H CPMG relaxation dispersion NMR spectroscopy is possible and can be applied to study functional motions on the microsecond time scale that cannot be accessed by backbone (15)N relaxation dispersion NMR. The use of methyl groups as dynamics probes extends such applications also to larger proteins.

Dataset Information

Complex B₁⁺ mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

Purpose

Methods

Results

Conclusion

Publications

Complex B<sub>1</sub><sup>+</sup> mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Dataset Information

Complex B1+ mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

Purpose

Methods

Results

Conclusion

Publications

Complex B<sub>1</sub><sup>+</sup> mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Complex B₁⁺ mapping with Carr-Purcell spin echoes and its application to electrical properties tomography.